چکیده:
Background. BDNF and NT-4/5 have been proposed to be involved in the coordinated adaptations of the neuromuscular system to the elevated level of activity, but an activity-dependent expression of neurotrophins in skeletal muscle is not well established.
Objectives. We, therefore, investigated the effect of one session of resistance exercise on mRNA expression of some neurotrophins in Slow and fast muscles of Wistar rats.
Methods. The resistance training protocol consisted of climbing a 1-meter–long ladder, with a weight attached to a tail sleeve. Twenty-four hours following the main training session, Soleus and Flexor Hallucis Longus (FHL) muscles were removed. mRNA expression of BDNF, NT4/5, TrkB and p75 proteins was assessed by Quantitative RT-PCR.
Results. The data analysis showed that one session of resistance exercise significantly (p<0.05) decreased mRNA expression of NT4/5 in soleus muscle, but not in FHL muscle. No significant effects of one resistance exercise bout were detected for BDNF and trkB. Our results also show that p75 mRNA levels in the soleus muscle were significantly elevated (7folds) after one resistance training bout (p<0.05).
Conclusion. The results indicate differential control of BDNF and NT-4/5 expression following resistance exercise in skeletal muscle. Also, we have provided evidence supporting the role of the p75 receptor in neurotrophins response to resistance exercise as a mechanical stimulus.
خلاصه ماشینی:
Recently, brief treadmilltraining bouts over 5 days were found to producelarge increases in BDNF mRNA in soleus muscle(2,6,10), but one treadmill exercise bout do noteffect on BDNF expression (2).
This data was demonstrated that effects ofexercise training on neurotrophins expression inskeletal muscle are not clear.
However, Expression of GAPDH was moststable following resistance exercise in both soleusand FHL muscles (p=0.
However, there wereno detectable changes in the level of BDNFmRNA in soleus and FHL muscles after onesession of resistance exercise (fig.
In our study wedetected BDNF mRNA in low content that limited48 Skeletal Muscle-Expressed Neurotrophins Response to Acute Resistance ExerciseEslami, R.
Effects of one session of resistance exercise on BDNF and NT-4/5 expression inFlexor Hallucis Longus (FHL) (filled bars) and soleus (open bars).
Effects of one session of resistance exercise on TrkB and p75 expression inFlexor Hallucis Longus (FHL) (filled bars) and soleus (open bars).
Although muscle-derived BDNF wasidentified as an activity-dependent factor, themost previous studies did not reveal effect ofsingle bout of exercise on BDNF expression(2,10).
Effects of exercise and muscle type on BDNF, NT-4/5, and TrkB expression in skeletalmuscle.
com at 12:15 +0330 on Saturday October 6th 2018Skeletal Muscle-Expressed Neurotrophins Response to Acute Resistance Exercise 53Eslami, R.
com at 12:15 +0330 on Saturday October 6th 2018Skeletal Muscle-Expressed Neurotrophins Response to Acute Resistance Exercise 53Eslami, R.
com at 12:15 +0330 on Saturday October 6th 2018Skeletal Muscle-Expressed Neurotrophins Response to Acute Resistance Exercise 53Eslami, R.
com at 12:15 +0330 on Saturday October 6th 2018Skeletal Muscle-Expressed Neurotrophins Response to Acute Resistance Exercise 53Eslami, R.